TGML service request

  - ChIP-seq:

        Recommended quantity: 0.05-50 ng of DNA

  - RNA-seq:

      - Poly(A)-selection :

        Recommended quantity: 0.025 - 1 µg of total RNA
        Volume: < 40 µL
        Sample Purity (DO260/280): 1.8 - 2.2 / RIN ≥ 8

      - Ribo-depletion:

        Recommended quantity: 1 - 5 µg of total RNA
        Volume: < 20 µL
        Sample Purity (DO260/280): 1.8 - 2.2 / RIN ≥ 8

      - miRNA-seq:

        Recommended quantity: 0.5 - 2 µg of total RNA
        Sample Purity (DO260/280): 1.8 - 2.2 / RIN ≥ 8

     - Sequencing with library preparation with Chromium Controller :

          + Single cell :

             Recommended quantity: depends on the project
             Sample quality: viability 90%, dissociated cells

  - Total RNA:

    Recommended quantity: 50 - 200 ng of total RNA
    Volume : < 20 µL
    Sample Purity (DO260/280): 1.8 - 2.2 / RIN ≥ 8

  - Poly(A)-RNA :

    Recommended quantity: 5 ng of poly(A)-RNA
    Volume: < 20 µL
    Sample Purity (DO260/280 : 1.8 - 2.2 / RIN ≥ 8

  - Small RNA:

    Recommended quantity: 500 ng of total RNA
    Concentration: 100 ng/µL
    Sample Purity (DO260/280): 1.8 - 2.2 / RIN ≥ 8