Date de l'évènement: Thursday, 20 September, 2018 to Thursday, 1 August, 2019

This project aim to dissect the role of Epromoters, a new type of regulatory elements discovered by the TAGC team, in the coordinated gene expression response to inflammation and stress signalling. The project will be led by Salvatore Spicuglia in collaboration with the laboratories of Eric Soler (IGMM, Montpellier), Cyril Esnault/Jean-Christophe Andrau (IGMM, Montpellier) and Michael Sieweke (CIML, Marseille). For additional information:
https://tagc.univ-amu.fr/en/users/spicuglia-salvatore#ANR%20Epromoter

Dissection of coordinated stress responses by novel promoter elements with enhancer activity

Funding: ANR

Collaborators: Cyril Esnault, Jean-Christophe Andrau and Eric Soler (IGMM, Montpellier); Sandrine Sarrazin and Michael Sieweke (CIML, Marseille)

A central dogma in the field of transcriptional regulation implies that gene expression is controlled by the involvement of gene-proximal (promoters) and distal (enhancers) regulatory elements. By combining an original high-throughput reporter assay and CRISPR genetic manipulations, we recently demonstrated that a subset of gene promoters, termed Epromoters, works “also” as bona fide enhancers and regulates distal gene expression. We hypothesized that Epromoters play a key role in the coordination of rapid gene induction in the stress response, in particular during inflammation. In the current project, we aim to study the functional role of Epromoters in the interferon and serum response, determine their impact in genome topology, and establish in vivo models to assess Epromoter variation in inflammatory diseases. Our systematic approach will unravel the basic molecular mechanisms of this novel type of regulatory elements and shed the basis of their potential involvement in diseases