TAGC's Molecular and Cellular Biology Facility is responsible for:

•  service activities: upkeep and maintenance of equipment, management of reagent and consumable stocks;
• training in the use of instruments and basic techniques in molecular and cellular biology, and skills transfer;
• technological development activities;
• experimental activities dedicated to TAGC research projects.

• Extraction, quantitative and qualitative analysis of nucleic acids (RNA and DNA),
• molecular cloning,
• conventional PCR,
• qPCR (genotyping by allelic discrimination, mDNA and gDNA quantification),
• qRT-PCR (SybGreen or hydrolysis probe),
• Dual-luciferase reporter gene test,
• EMSA,
• preparation of libraries for ChIP-seq,
• sample preparation for RNA-seq or scRAN-seq,
• CRISPR (KO, KI, interference or activation),
• MPRA (Massively Parallel Reporter Assay)

Sample quality analysis and quantification

qPCR instruments

Imaging

Cell culture