- ChIP-seq:
Recommended quantity: 0.05-50 ng of DNA
- RNA-seq:
- Poly(A)-selection :
Recommended quantity: 0.025 - 1 µg of total RNA Volume: < 40 µL Sample Purity (DO260/280): 1.8 - 2.2 / RIN ≥ 8
- Ribo-depletion:
Recommended quantity: 1 - 5 µg of total RNA Volume: < 20 µL Sample Purity (DO260/280): 1.8 - 2.2 / RIN ≥ 8
- miRNA-seq:
Recommended quantity: 0.5 - 2 µg of total RNA Sample Purity (DO260/280): 1.8 - 2.2 / RIN ≥ 8
- Sequencing with library preparation with Chromium Controller :
+ Single cell :
Recommended quantity: depends on the project Sample quality: viability 90%, dissociated cells
- Total RNA:
Recommended quantity: 50 - 200 ng of total RNA Volume : < 20 µL Sample Purity (DO260/280): 1.8 - 2.2 / RIN ≥ 8
- Poly(A)-RNA :
Recommended quantity: 5 ng of poly(A)-RNA Volume: < 20 µL Sample Purity (DO260/280 : 1.8 - 2.2 / RIN ≥ 8
- Small RNA:
Recommended quantity: 500 ng of total RNA Concentration: 100 ng/µL Sample Purity (DO260/280): 1.8 - 2.2 / RIN ≥ 8
