Our main missions
TAGC's Molecular and Cellular Biology Facility is responsible for:
- service activities: upkeep and maintenance of equipment, management of reagent and consumable stocks;
- training in the use of instruments and basic techniques in molecular and cellular biology, and skills transfer;
- technological development activities;
- experimental activities dedicated to TAGC research projects.
|
|
|
Techniques and technological approaches developed
- Extraction, quantitative and qualitative analysis of nucleic acids (RNA and DNA),
- molecular cloning,
- conventional PCR,
- qPCR (genotyping by allelic discrimination, mDNA and gDNA quantification),
- qRT-PCR (SybGreen or hydrolysis probe),
- Dual-luciferase reporter gene test,
- EMSA,
- ChIP,
- preparation of libraries for RNA-seq or scRAN-seq,
- CRISPR (KO, KI, interference or activation),
- MPRA (Massively Parallel Reporter Assay)
|
|
Instruments
Sample quality analysis and quantification
|
|
|
|
|
qPCR instruments
|
|
|
|
|
Imaging
|
|
|
|
|
Cell culture
|
|
|
|
|
List of publications
"""" Trouver moyen de mettre un facette publication de publi choisies à partir de Hal id comme sur annuaire """"