Our main missions

TAGC's Molecular and Cellular Biology Facility is responsible for:

  •  service activities: upkeep and maintenance of equipment, management of reagent and consumable stocks;
  • training in the use of instruments and basic techniques in molecular and cellular biology, and skills transfer;
  • technological development activities;
  • experimental activities dedicated to TAGC research projects.

 

 

Techniques and technological approaches developed

  • Extraction, quantitative and qualitative analysis of nucleic acids (RNA and DNA),
  • molecular cloning,
  • conventional PCR,
  • qPCR (genotyping by allelic discrimination, mDNA and gDNA quantification),
  • qRT-PCR (SybGreen or hydrolysis probe),
  • Dual-luciferase reporter gene test,
  • EMSA,
  • preparation of libraries for ChIP-seq,
  • sample preparation for RNA-seq or scRAN-seq,
  • CRISPR (KO, KI, interference or activation),
  • MPRA (Massively Parallel Reporter Assay)

 

Instruments

Sample quality analysis and quantification
Bioanalyzer 2100, Agilent
Nanodrop, nano-volume spectrophotometer
Qubit, fluorometer
Nivo Victor plate reader
qPCR instruments
6Flex, Applied Biosystems
QuantStudio3, Thermo Fisher Scientific
AriaMx, Agilent
 
Imaging
imageur iBright CL750, Thermo Fisher Scientific
EVOS M3000 imaging system
   
Cell culture
NEON transfection system
COUNTESS cell counter
Precellys tissue grinder homogenizer
 

 

List of publications 

hal-03162042v1  Journal article
 Rare Pathogenic Variants in Mitochondrial and Inflammation-Associated Genes May Lead to Inflammatory Cardiomyopathy in Chagas Disease
Journal of Clinical Immunology, 2021, ⟨10.1007/s10875-021-01000-y⟩
hal-04549763v1  Journal article
 From Genome-wide Association Studies to Functional Variants: ARL14 Cis - regulatory Variants Are Associated With Severe Malaria
Journal of Infectious Diseases, 2024, pp.jiae159. ⟨10.1093/infdis/jiae159⟩
hal-04268017v1  Journal article
 A Non-Coding Fc Gamma Receptor Cis-Regulatory Variant within the 1q23 Gene Cluster Is Associated with Plasmodium falciparum Infection in Children Residing in Burkina Faso
International Journal of Molecular Sciences, 2023, 24 (21), pp.15711. ⟨10.3390/ijms242115711⟩
hal-04044391v1  Journal article
 Short tandem repeats are important contributors to silencer elements in T cells
Nucleic Acids Research, 2023, 51 (10), pp.4845-4866. ⟨10.1093/nar/gkad187⟩
hal-03513096v1  Journal article
 Co-Exposure of Cardiomyocytes to IFN-γ and TNF-α Induces Mitochondrial Dysfunction and Nitro-Oxidative Stress: Implications for the Pathogenesis of Chronic Chagas Disease Cardiomyopathy
Frontiers in Immunology, 2021, 12, ⟨10.3389/fimmu.2021.755862⟩
hal-03690258v1  Journal article
 Identification of ATP2B4 Regulatory Element Containing Functional Genetic Variants Associated with Severe Malaria
International Journal of Molecular Sciences, 2022, 23 (9), pp.4849. ⟨10.3390/ijms23094849⟩
hal-04750444v1  Journal article
 An Expression Quantitative Trait Locus of Fc Gamma Receptor Genes is Associated with Anti-Malarial IgG Responses and Infection Levels in Burkinabe Families Running Title: Association of rs1771575 with Malaria
Journal of Infectious Diseases, In press, 2231 (4), pp.1008-1019. ⟨10.1093/infdis/jiae528⟩

 

Team members